CRISPR関連生体分子データ

◆ CRISPR関連生体分子データ ◆
＝ゲノム編集を考える＝

Jmol分子表示（Cas9，Cpf1）｜ CRISPR-Cas9情報｜ CRISPR-Cpf1情報｜ニュース

ゲノム編集研究でEmmanuelle CharpentierとJennifer A. Doudnaが2020年ノーベル化学賞を受賞しました！　→ 関連記事
 「生物の科学遺伝」2018年11月発刊号に特集『ゲノム編集』が掲載されました。 → PDF版販売 2017/04/02に新潟市内で第98回サイエンスカフェにいがた『“ゲノム編集”が世界を変える？』を開催しました。
ゲノム編集研究が日本国際賞を受賞（2017/02/03公表）： 2017年（第33回）Japan Prize受賞者　「生命科学」分野　CRISPR-Casによるゲノム編集機構の解明（国際科学技術財団）　→ #ゲノム編集。CRISPR。ゲノム編集研究が日本国際賞受賞 - Togetterまとめ
本ページについて日本コンピュータ化学会2016春季年会（2016/06/02-03，東京工業大学大岡山キャンパス；プログラム）でポスター発表しました → 1P09要旨 [PDF]

初期表示はPDBデータ 5f9r（sgRNAおよび2本鎖DNAと結合したCRISPR-Cas9；2020年ノーベル化学賞受賞のJ.A.Doudnaらによる）
※Jmolによる分子表示はWindows 10でもInternet Explorer 11により可能です。

■CRISPR-Cas9（Cas9以外のCasも含む）
4ogc（Cas9タンパク質；2020年ノーベル化学賞受賞のJ.A.Doudnaらによる）　SPD（スペルミジン）選択　同PDBsumデータ 4ogc_SPD$　→ Science論文(2014)
4oo8のChain A・B・C（Cas9タンパク質-ガイド鎖RNA-標的DNAの複合体）
4qyz（標的ssDNA-Casタンパク質-crRNA複合体）
4un3（PAM（protospacer adjacent motif）を含む標的DNAと結合したCas9エンドヌクレアーゼ）　→ 3Dプリンタ模型情報
5b2o（Cas9タンパク質-sgRNA-標的DNAの複合体；TGG PAM）　同Chain B-D（sgRNA-標的DNAのみ）｜ 5b2p（同；TGA PAM）同Chain B-D（sgRNA-標的DNAのみ）｜ 5b2q（同；TGG PAM）同Chain B-D（sgRNA-標的DNAのみ） ∥ 5b2r（同；TGA PAM）｜ 5b2s（同；TGAG PAM）｜ 5b2t（同；TGCG PAM）
5f9r（sgRNAおよび2本鎖DNAと結合したCRISPR-Cas9；2020年ノーベル化学賞受賞のJ.A.Doudnaらによる）
5fq5（Cas9-sgRNA-DNA複合体；SAD法〈単波長異常散乱法〉による）
5fw1（SpyCas9-sgRNA-標的DNA複合体；TGAG PAM）｜ 5fw2（同；TGAG PAM）｜ 5fw3（同；TGCG PAM）
5xbl（SpyCas9-sgRNA複合体；抗CRISPRタンパク質AcrIIA4が結合）
5h9f（標的dsDNA-Casタンパク質-crRNA複合体；5'-ATG PAMを認識）　23G（guanosine-5'-phosphate-2',3'-cyclic phosphate）選択
5x2g（標的DNA-Casタンパク質-sgRNA複合体；AGAAACC PAM）｜ 5x2h（同；AGAAACA PAM）
4ic1（Cas4タンパク質）　→ Jmolトピック
6thh（Cas10dタンパク質；抗CRISPRタンパク質AcrID1が結合）　→ Jmolトピック
5nfv（Cpf1(Cas12a)タンパク質-crRNA-標的DNA複合体）｜ 5ng6のChain A・B（Cpf1(Cas12a)タンパク質-crRNA複合体）
7c7l（Cas12fタンパク質）
8h1j（TnpBタンパク質〈Cas12ファミリー〉-ωRNA-標的DNA複合体）
7vtn（Cas13bt3タンパク質-crRNA-標的DNA複合体）
6e79（CasX-gRNA-DNA複合体）
5b43（Cpf1タンパク質-crRNA-標的DNA複合体；5'-TTTN-3' PAM）
5mga（Cpf1タンパク質-crRNA-DNA複合体）
5xh6（Cpf1タンパク質-crRNA-標的DNA複合体；TATA PAM）｜ 5xh7（Cpf1タンパク質-crRNA-標的DNA複合体；TCCA PAM）
■その他
ベムラフェニブ（vemurafenib；悪性黒色腫の治療薬，WIRED.jp記事参照）　※参考（ベムラフェニブ結合PDBデータ例： 3og7（B-Rafプロテインキナーゼ）　同PDBsumデータ 3og7_032$　→ Jmolトピック

バックボーン　二次構造　DNA/RNA（ATGCU；塩基のみ）　DNA/RNA（ATGCU，backbone）
全選択　タンパク質選択　リガンド選択　DNA/RNA選択　DNA選択　DNA/RNA塩基選択　DNA/RNA backbone選択　水分子表示
Chain A選択　B選択　C選択　D選択
空間充填　球棒　球30％　球60％　スティック　針金　消去
アミノ色　Chain色　CPK色　∥　Jmol色　Rasmol色
リガンドの元素記号表示
酸性・中性・塩基性区別　極性・非極性区別
疎水性インデックス順　I/O値順（特性基 R）　等電点順
コンホメーション選択性（αヘリックス・βストランド）
水素結合表示
S-S結合表示（太）　S原子（Protein中）空間充填
ドット表面表示　α炭素ラベル表示　回転
背景・黒　灰　白　紺

ASP　GLU　CYS　MET　LYS　ARG　SER　THR　PHE　TYR　ASN　GLN　GLY　LEU　VAL　ILE　ALA　TRP　HIS　PRO

ASP　GLU　GLY　ALA　VAL　LEU　ILE　CYS　SER　THR　ASN　GLN　PRO　MET　PHE　TYR　TRP　LYS　ARG　HIS

SER　THR　TYR　CYS　ASN　GLN　ASP　GLU　LYS　ARG　HIS　GLY　ALA　VAL　LEU　ILE　PHE　PRO　MET　TRP

ARG　LYS　ASN　ASP　GLN　GLU　HIS　PRO　TYR　TRP　SER　THR　GLY　ALA　MET　CYS　PHE　LEU　VAL　ILE

ASN　SER　ASP　GLN　GLU　THR　ARG　HIS　GLY　LYS　TYR　TRP　CYS　MET　PRO　PHE　ALA　VAL　LEU　ILE

ASP　GLU　CYS　ASN　PHE　GLN　TYR　SER　MET　TRP　VAL　GLY　LEU　ALA　ILE　THR　PRO　HIS　LYS　ARG

アミノ酸および特性基の親水性・疎水性｜ Log Pをポケットに！

■CRISPR-Cas9（Cas9以外のCasも含む）

Cas9タンパク質4ogcとそのPDBsumデータ（Science論文(2014)；2020年ノーベル化学賞受賞のJ.A.Doudnaらによる）

［左］5f9r（2020年ノーベル化学賞受賞のJ.A.Doudnaらによる）　［右］4oo8のChain A-C ；・

［左］4qyz（空間充填がcrRNA，小さめの球表示が標的ssDNA）　［中］5h9f　［右］5h9fと同データ引用Nature論文掲載図との比較

左から 4un3（空間充填が標的DNA，小さめの球表示がsgRNA），同3Dプリンタ模型（3dmoleculardesigns.com），
同データをより作成した画像が表紙の「生物の科学遺伝」2018年11月発刊号（2021年発刊号 p.8にも掲載），The Mercury News記事（2018/09/10）

［左］5b2q
［右］同Chain B-D（sgRNA-標的DNAのみ；スティック表示がRNAで球棒表示がDNA〈塩基部分だけドット表面表示〉）　…クリックで拡大

5b2r
※recognition lobe (REC)，nuclease lobe (NUC)についてはCas9 - Wikipedia，Cell資料（2014/06/05） [PDF]ほか参照

5fw1（左）と5fw3（右）

5x2g（左）と5x2h（右）

［左］抗CRISPRタンパク質AcrIIA4が結合したSpyCas9-sgRNA複合体5xbl　…Nature ハイライト（2017/06/15）参照
［右］抗CRISPRタンパク質AcrID1が結合したCas10dタンパク質6thh　→ Jmolトピック

Cas12f-gRNA-標的DNA複合体 7c7l

TnpBタンパク質（Cas12ファミリー）-ωRNA-標的DNA複合体8h1j

Cas13bt3（Cas13X.1）タンパク質-crRNA-標的DNA複合体 7vtn

CasX-gRNA-DNA複合体 6e79

【参考】Cas9タンパク質のアミノ酸配列比較（FASTA形式データから作成）；4oo8（B鎖）と5f9r（A鎖，2020年ノーベル化学賞受賞のJ.A.Doudnaらによる）　　↓ 後者を4つ後ろに動かすと…

001-050
	4oo8	Gly	Ser	Gly	His	Met	Asp	Lys	Lys	Tyr	Ser	Ile	Gly	Leu	Ala	Ile	Gly	Thr	Asn	Ser	Val	Gly	Trp	Ala	Val	Ile	Thr	Asp	Glu	Tyr	Lys	Val	Pro	Ser	Lys	Lys	Phe	Lys	Val	Leu	Gly	Asn	Thr	Asp	Arg	His	Ser	Ile	Lys	Lys	Asn
	5f9r	Met	Asp	Lys	Lys	Tyr	Ser	Ile	Gly	Leu	Asp	Ile	Gly	Thr	Asn	Ser	Val	Gly	Trp	Ala	Val	Ile	Thr	Asp	Glu	Tyr	Lys	Val	Pro	Ser	Lys	Lys	Phe	Lys	Val	Leu	Gly	Asn	Thr	Asp	Arg	His	Ser	Ile	Lys	Lys	Asn	Leu	Ile	Gly	Ala
051-100
	4oo8	Leu	Ile	Gly	Ala	Leu	Leu	Phe	Asp	Ser	Gly	Glu	Thr	Ala	Glu	Ala	Thr	Arg	Leu	Lys	Arg	Thr	Ala	Arg	Arg	Arg	Tyr	Thr	Arg	Arg	Lys	Asn	Arg	Ile	Leu	Tyr	Leu	Gln	Glu	Ile	Phe	Ser	Asn	Glu	Met	Ala	Lys	Val	Asp	Asp	Ser
	5f9r	Leu	Leu	Phe	Asp	Ser	Gly	Glu	Thr	Ala	Glu	Ala	Thr	Arg	Leu	Lys	Arg	Thr	Ala	Arg	Arg	Arg	Tyr	Thr	Arg	Arg	Lys	Asn	Arg	Ile	Cys	Tyr	Leu	Gln	Glu	Ile	Phe	Ser	Asn	Glu	Met	Ala	Lys	Val	Asp	Asp	Ser	Phe	Phe	His	Arg
101-150
	4oo8	Phe	Phe	His	Arg	Leu	Glu	Glu	Ser	Phe	Leu	Val	Glu	Glu	Asp	Lys	Lys	His	Glu	Arg	His	Pro	Ile	Phe	Gly	Asn	Ile	Val	Asp	Glu	Val	Ala	Tyr	His	Glu	Lys	Tyr	Pro	Thr	Ile	Tyr	His	Leu	Arg	Lys	Lys	Leu	Val	Asp	Ser	Thr
	5f9r	Leu	Glu	Glu	Ser	Phe	Leu	Val	Glu	Glu	Asp	Lys	Lys	His	Glu	Arg	His	Pro	Ile	Phe	Gly	Asn	Ile	Val	Asp	Glu	Val	Ala	Tyr	His	Glu	Lys	Tyr	Pro	Thr	Ile	Tyr	His	Leu	Arg	Lys	Lys	Leu	Val	Asp	Ser	Thr	Asp	Lys	Ala	Asp
151-200
	4oo8	Asp	Lys	Ala	Asp	Leu	Arg	Leu	Ile	Tyr	Leu	Ala	Leu	Ala	His	Met	Ile	Lys	Phe	Arg	Gly	His	Phe	Leu	Ile	Glu	Gly	Asp	Leu	Asn	Pro	Asp	Asn	Ser	Asp	Val	Asp	Lys	Leu	Phe	Ile	Gln	Leu	Val	Gln	Thr	Tyr	Asn	Gln	Leu	Phe
	5f9r	Leu	Arg	Leu	Ile	Tyr	Leu	Ala	Leu	Ala	His	Met	Ile	Lys	Phe	Arg	Gly	His	Phe	Leu	Ile	Glu	Gly	Asp	Leu	Asn	Pro	Asp	Asn	Ser	Asp	Val	Asp	Lys	Leu	Phe	Ile	Gln	Leu	Val	Gln	Thr	Tyr	Asn	Gln	Leu	Phe	Glu	Glu	Asn	Pro
201-250
	4oo8	Glu	Glu	Asn	Pro	Ile	Asn	Ala	Ser	Gly	Val	Asp	Ala	Lys	Ala	Ile	Leu	Ser	Ala	Arg	Leu	Ser	Lys	Ser	Arg	Arg	Leu	Glu	Asn	Leu	Ile	Ala	Gln	Leu	Pro	Gly	Glu	Lys	Lys	Asn	Gly	Leu	Phe	Gly	Asn	Leu	Ile	Ala	Leu	Ser	Leu
	5f9r	Ile	Asn	Ala	Ser	Gly	Val	Asp	Ala	Lys	Ala	Ile	Leu	Ser	Ala	Arg	Leu	Ser	Lys	Ser	Arg	Arg	Leu	Glu	Asn	Leu	Ile	Ala	Gln	Leu	Pro	Gly	Glu	Lys	Lys	Asn	Gly	Leu	Phe	Gly	Asn	Leu	Ile	Ala	Leu	Ser	Leu	Gly	Leu	Thr	Pro
251-300
	4oo8	Gly	Leu	Thr	Pro	Asn	Phe	Lys	Ser	Asn	Phe	Asp	Leu	Ala	Glu	Asp	Ala	Lys	Leu	Gln	Leu	Ser	Lys	Asp	Thr	Tyr	Asp	Asp	Asp	Leu	Asp	Asn	Leu	Leu	Ala	Gln	Ile	Gly	Asp	Gln	Tyr	Ala	Asp	Leu	Phe	Leu	Ala	Ala	Lys	Asn	Leu
	5f9r	Asn	Phe	Lys	Ser	Asn	Phe	Asp	Leu	Ala	Glu	Asp	Ala	Lys	Leu	Gln	Leu	Ser	Lys	Asp	Thr	Tyr	Asp	Asp	Asp	Leu	Asp	Asn	Leu	Leu	Ala	Gln	Ile	Gly	Asp	Gln	Tyr	Ala	Asp	Leu	Phe	Leu	Ala	Ala	Lys	Asn	Leu	Ser	Asp	Ala	Ile
301-350
	4oo8	Ser	Asp	Ala	Ile	Leu	Leu	Ser	Asp	Ile	Leu	Arg	Val	Asn	Thr	Glu	Ile	Thr	Lys	Ala	Pro	Leu	Ser	Ala	Ser	Met	Ile	Lys	Arg	Tyr	Asp	Glu	His	His	Gln	Asp	Leu	Thr	Leu	Leu	Lys	Ala	Leu	Val	Arg	Gln	Gln	Leu	Pro	Glu	Lys
	5f9r	Leu	Leu	Ser	Asp	Ile	Leu	Arg	Val	Asn	Thr	Glu	Ile	Thr	Lys	Ala	Pro	Leu	Ser	Ala	Ser	Met	Ile	Lys	Arg	Tyr	Asp	Glu	His	His	Gln	Asp	Leu	Thr	Leu	Leu	Lys	Ala	Leu	Val	Arg	Gln	Gln	Leu	Pro	Glu	Lys	Tyr	Lys	Glu	Ile
351-400
	4oo8	Tyr	Lys	Glu	Ile	Phe	Phe	Asp	Gln	Ser	Lys	Asn	Gly	Tyr	Ala	Gly	Tyr	Ile	Asp	Gly	Gly	Ala	Ser	Gln	Glu	Glu	Phe	Tyr	Lys	Phe	Ile	Lys	Pro	Ile	Leu	Glu	Lys	Met	Asp	Gly	Thr	Glu	Glu	Leu	Leu	Val	Lys	Leu	Asn	Arg	Glu
	5f9r	Phe	Phe	Asp	Gln	Ser	Lys	Asn	Gly	Tyr	Ala	Gly	Tyr	Ile	Asp	Gly	Gly	Ala	Ser	Gln	Glu	Glu	Phe	Tyr	Lys	Phe	Ile	Lys	Pro	Ile	Leu	Glu	Lys	Met	Asp	Gly	Thr	Glu	Glu	Leu	Leu	Val	Lys	Leu	Asn	Arg	Glu	Asp	Leu	Leu	Arg
401-450
	4oo8	Asp	Leu	Leu	Arg	Lys	Gln	Arg	Thr	Phe	Asp	Asn	Gly	Ser	Ile	Pro	His	Gln	Ile	His	Leu	Gly	Glu	Leu	His	Ala	Ile	Leu	Arg	Arg	Gln	Glu	Asp	Phe	Tyr	Pro	Phe	Leu	Lys	Asp	Asn	Arg	Glu	Lys	Ile	Glu	Lys	Ile	Leu	Thr	Phe
	5f9r	Lys	Gln	Arg	Thr	Phe	Asp	Asn	Gly	Ser	Ile	Pro	His	Gln	Ile	His	Leu	Gly	Glu	Leu	His	Ala	Ile	Leu	Arg	Arg	Gln	Glu	Asp	Phe	Tyr	Pro	Phe	Leu	Lys	Asp	Asn	Arg	Glu	Lys	Ile	Glu	Lys	Ile	Leu	Thr	Phe	Arg	Ile	Pro	Tyr
451-500
	4oo8	Arg	Ile	Pro	Tyr	Tyr	Val	Gly	Pro	Leu	Ala	Arg	Gly	Asn	Ser	Arg	Phe	Ala	Trp	Met	Thr	Arg	Lys	Ser	Glu	Glu	Thr	Ile	Thr	Pro	Trp	Asn	Phe	Glu	Glu	Val	Val	Asp	Lys	Gly	Ala	Ser	Ala	Gln	Ser	Phe	Ile	Glu	Arg	Met	Thr
	5f9r	Tyr	Val	Gly	Pro	Leu	Ala	Arg	Gly	Asn	Ser	Arg	Phe	Ala	Trp	Met	Thr	Arg	Lys	Ser	Glu	Glu	Thr	Ile	Thr	Pro	Trp	Asn	Phe	Glu	Glu	Val	Val	Asp	Lys	Gly	Ala	Ser	Ala	Gln	Ser	Phe	Ile	Glu	Arg	Met	Thr	Asn	Phe	Asp	Lys
501-550
	4oo8	Asn	Phe	Asp	Lys	Asn	Leu	Pro	Asn	Glu	Lys	Val	Leu	Pro	Lys	His	Ser	Leu	Leu	Tyr	Glu	Tyr	Phe	Thr	Val	Tyr	Asn	Glu	Leu	Thr	Lys	Val	Lys	Tyr	Val	Thr	Glu	Gly	Met	Arg	Lys	Pro	Ala	Phe	Leu	Ser	Gly	Glu	Gln	Lys	Lys
	5f9r	Asn	Leu	Pro	Asn	Glu	Lys	Val	Leu	Pro	Lys	His	Ser	Leu	Leu	Tyr	Glu	Tyr	Phe	Thr	Val	Tyr	Asn	Glu	Leu	Thr	Lys	Val	Lys	Tyr	Val	Thr	Glu	Gly	Met	Arg	Lys	Pro	Ala	Phe	Leu	Ser	Gly	Glu	Gln	Lys	Lys	Ala	Ile	Val	Asp
551-600
	4oo8	Ala	Ile	Val	Asp	Leu	Leu	Phe	Lys	Thr	Asn	Arg	Lys	Val	Thr	Val	Lys	Gln	Leu	Lys	Glu	Asp	Tyr	Phe	Lys	Lys	Ile	Glu	Glu	Phe	Asp	Ser	Val	Glu	Ile	Ser	Gly	Val	Glu	Asp	Arg	Phe	Asn	Ala	Ser	Leu	Gly	Thr	Tyr	His	Asp
	5f9r	Leu	Leu	Phe	Lys	Thr	Asn	Arg	Lys	Val	Thr	Val	Lys	Gln	Leu	Lys	Glu	Asp	Tyr	Phe	Lys	Lys	Ile	Glu	Cys	Phe	Asp	Ser	Val	Glu	Ile	Ser	Gly	Val	Glu	Asp	Arg	Phe	Asn	Ala	Ser	Leu	Gly	Thr	Tyr	His	Asp	Leu	Leu	Lys	Ile
601-650
	4oo8	Leu	Leu	Lys	Ile	Ile	Lys	Asp	Lys	Asp	Phe	Leu	Asp	Asn	Glu	Glu	Asn	Glu	Asp	Ile	Leu	Glu	Asp	Ile	Val	Leu	Thr	Leu	Thr	Leu	Phe	Glu	Asp	Arg	Glu	Met	Ile	Glu	Glu	Arg	Leu	Lys	Thr	Tyr	Ala	His	Leu	Phe	Asp	Asp	Lys
	5f9r	Ile	Lys	Asp	Lys	Asp	Phe	Leu	Asp	Asn	Glu	Glu	Asn	Glu	Asp	Ile	Leu	Glu	Asp	Ile	Val	Leu	Thr	Leu	Thr	Leu	Phe	Glu	Asp	Arg	Glu	Met	Ile	Glu	Glu	Arg	Leu	Lys	Thr	Tyr	Ala	His	Leu	Phe	Asp	Asp	Lys	Val	Met	Lys	Gln
651-700
	4oo8	Val	Met	Lys	Gln	Leu	Lys	Arg	Arg	Arg	Tyr	Thr	Gly	Trp	Gly	Arg	Leu	Ser	Arg	Lys	Leu	Ile	Asn	Gly	Ile	Arg	Asp	Lys	Gln	Ser	Gly	Lys	Thr	Ile	Leu	Asp	Phe	Leu	Lys	Ser	Asp	Gly	Phe	Ala	Asn	Arg	Asn	Phe	Met	Gln	Leu
	5f9r	Leu	Lys	Arg	Arg	Arg	Tyr	Thr	Gly	Trp	Gly	Arg	Leu	Ser	Arg	Lys	Leu	Ile	Asn	Gly	Ile	Arg	Asp	Lys	Gln	Ser	Gly	Lys	Thr	Ile	Leu	Asp	Phe	Leu	Lys	Ser	Asp	Gly	Phe	Ala	Asn	Arg	Asn	Phe	Met	Gln	Leu	Ile	His	Asp	Asp
701-750
	4oo8	Ile	His	Asp	Asp	Ser	Leu	Thr	Phe	Lys	Glu	Asp	Ile	Gln	Lys	Ala	Gln	Val	Ser	Gly	Gln	Gly	Asp	Ser	Leu	His	Glu	His	Ile	Ala	Asn	Leu	Ala	Gly	Ser	Pro	Ala	Ile	Lys	Lys	Gly	Ile	Leu	Gln	Thr	Val	Lys	Val	Val	Asp	Glu
	5f9r	Ser	Leu	Thr	Phe	Lys	Glu	Asp	Ile	Gln	Lys	Ala	Gln	Val	Ser	Gly	Gln	Gly	Asp	Ser	Leu	His	Glu	His	Ile	Ala	Asn	Leu	Ala	Gly	Ser	Pro	Ala	Ile	Lys	Lys	Gly	Ile	Leu	Gln	Thr	Val	Lys	Val	Val	Asp	Glu	Leu	Val	Lys	Val
751-800
	4oo8	Leu	Val	Lys	Val	Met	Gly	Arg	His	Lys	Pro	Glu	Asn	Ile	Val	Ile	Glu	Met	Ala	Arg	Glu	Asn	Gln	Thr	Thr	Gln	Lys	Gly	Gln	Lys	Asn	Ser	Arg	Glu	Arg	Met	Lys	Arg	Ile	Glu	Glu	Gly	Ile	Lys	Glu	Leu	Gly	Ser	Gln	Ile	Leu
	5f9r	Met	Gly	Arg	His	Lys	Pro	Glu	Asn	Ile	Val	Ile	Glu	Met	Ala	Arg	Glu	Asn	Gln	Thr	Thr	Gln	Lys	Gly	Gln	Lys	Asn	Ser	Arg	Glu	Arg	Met	Lys	Arg	Ile	Glu	Glu	Gly	Ile	Lys	Glu	Leu	Gly	Ser	Gln	Ile	Leu	Lys	Glu	His	Pro
801-850
	4oo8	Lys	Glu	His	Pro	Val	Glu	Asn	Thr	Gln	Leu	Gln	Asn	Glu	Lys	Leu	Tyr	Leu	Tyr	Tyr	Leu	Gln	Asn	Gly	Arg	Asp	Met	Tyr	Val	Asp	Gln	Glu	Leu	Asp	Ile	Asn	Arg	Leu	Ser	Asp	Tyr	Asp	Val	Asp	Ala	Ile	Val	Pro	Gln	Ser	Phe
	5f9r	Val	Glu	Asn	Thr	Gln	Leu	Gln	Asn	Glu	Lys	Leu	Tyr	Leu	Tyr	Tyr	Leu	Gln	Asn	Gly	Arg	Asp	Met	Tyr	Val	Asp	Gln	Glu	Leu	Asp	Ile	Asn	Arg	Leu	Ser	Asp	Tyr	Asp	Val	Asp	His	Ile	Val	Pro	Gln	Ser	Phe	Leu	Lys	Asp	Asp
851-900
	4oo8	Leu	Lys	Asp	Asp	Ser	Ile	Asp	Asn	Lys	Val	Leu	Thr	Arg	Ser	Asp	Lys	Asn	Arg	Gly	Lys	Ser	Asp	Asn	Val	Pro	Ser	Glu	Glu	Val	Val	Lys	Lys	Met	Lys	Asn	Tyr	Trp	Arg	Gln	Leu	Leu	Asn	Ala	Lys	Leu	Ile	Thr	Gln	Arg	Lys
	5f9r	Ser	Ile	Asp	Asn	Lys	Val	Leu	Thr	Arg	Ser	Asp	Lys	Asn	Arg	Gly	Lys	Ser	Asp	Asn	Val	Pro	Ser	Glu	Glu	Val	Val	Lys	Lys	Met	Lys	Asn	Tyr	Trp	Arg	Gln	Leu	Leu	Asn	Ala	Lys	Leu	Ile	Thr	Gln	Arg	Lys	Phe	Asp	Asn	Leu
901-950
	4oo8	Phe	Asp	Asn	Leu	Thr	Lys	Ala	Glu	Arg	Gly	Gly	Leu	Ser	Glu	Leu	Asp	Lys	Ala	Gly	Phe	Ile	Lys	Arg	Gln	Leu	Val	Glu	Thr	Arg	Gln	Ile	Thr	Lys	His	Val	Ala	Gln	Ile	Leu	Asp	Ser	Arg	Met	Asn	Thr	Lys	Tyr	Asp	Glu	Asn
	5f9r	Thr	Lys	Ala	Glu	Arg	Gly	Gly	Leu	Ser	Glu	Leu	Asp	Lys	Ala	Gly	Phe	Ile	Lys	Arg	Gln	Leu	Val	Glu	Thr	Arg	Gln	Ile	Thr	Lys	His	Val	Ala	Gln	Ile	Leu	Asp	Ser	Arg	Met	Asn	Thr	Lys	Tyr	Asp	Glu	Asn	Asp	Lys	Leu	Ile
951-1000
	4oo8	Asp	Lys	Leu	Ile	Arg	Glu	Val	Lys	Val	Ile	Thr	Leu	Lys	Ser	Lys	Leu	Val	Ser	Asp	Phe	Arg	Lys	Asp	Phe	Gln	Phe	Tyr	Lys	Val	Arg	Glu	Ile	Asn	Asn	Tyr	His	His	Ala	His	Asp	Ala	Tyr	Leu	Asn	Ala	Val	Val	Gly	Thr	Ala
	5f9r	Arg	Glu	Val	Lys	Val	Ile	Thr	Leu	Lys	Ser	Lys	Leu	Val	Ser	Asp	Phe	Arg	Lys	Asp	Phe	Gln	Phe	Tyr	Lys	Val	Arg	Glu	Ile	Asn	Asn	Tyr	His	His	Ala	His	Asp	Ala	Tyr	Leu	Asn	Ala	Val	Val	Gly	Thr	Ala	Leu	Ile	Lys	Lys
1001-1050
	4oo8	Leu	Ile	Lys	Lys	Tyr	Pro	Lys	Leu	Glu	Ser	Glu	Phe	Val	Tyr	Gly	Asp	Tyr	Lys	Val	Tyr	Asp	Val	Arg	Lys	Met	Ile	Ala	Lys	Ser	Glu	Gln	Glu	Ile	Gly	Lys	Ala	Thr	Ala	Lys	Tyr	Phe	Phe	Tyr	Ser	Asn	Ile	Met	Asn	Phe	Phe
	5f9r	Tyr	Pro	Lys	Leu	Glu	Ser	Glu	Phe	Val	Tyr	Gly	Asp	Tyr	Lys	Val	Tyr	Asp	Val	Arg	Lys	Met	Ile	Ala	Lys	Ser	Glu	Gln	Glu	Ile	Gly	Lys	Ala	Thr	Ala	Lys	Tyr	Phe	Phe	Tyr	Ser	Asn	Ile	Met	Asn	Phe	Phe	Lys	Thr	Glu	Ile
1051-1100
	4oo8	Lys	Thr	Glu	Ile	Thr	Leu	Ala	Asn	Gly	Glu	Ile	Arg	Lys	Arg	Pro	Leu	Ile	Glu	Thr	Asn	Gly	Glu	Thr	Gly	Glu	Ile	Val	Trp	Asp	Lys	Gly	Arg	Asp	Phe	Ala	Thr	Val	Arg	Lys	Val	Leu	Ser	Met	Pro	Gln	Val	Asn	Ile	Val	Lys
	5f9r	Thr	Leu	Ala	Asn	Gly	Glu	Ile	Arg	Lys	Arg	Pro	Leu	Ile	Glu	Thr	Asn	Gly	Glu	Thr	Gly	Glu	Ile	Val	Trp	Asp	Lys	Gly	Arg	Asp	Phe	Ala	Thr	Val	Arg	Lys	Val	Leu	Ser	Met	Pro	Gln	Val	Asn	Ile	Val	Lys	Lys	Thr	Glu	Val
1101-1150
	4oo8	Lys	Thr	Glu	Val	Gln	Thr	Gly	Gly	Phe	Ser	Lys	Glu	Ser	Ile	Leu	Pro	Lys	Arg	Asn	Ser	Asp	Lys	Leu	Ile	Ala	Arg	Lys	Lys	Asp	Trp	Asp	Pro	Lys	Lys	Tyr	Gly	Gly	Phe	Asp	Ser	Pro	Thr	Val	Ala	Tyr	Ser	Val	Leu	Val	Val
	5f9r	Gln	Thr	Gly	Gly	Phe	Ser	Lys	Glu	Ser	Ile	Leu	Pro	Lys	Arg	Asn	Ser	Asp	Lys	Leu	Ile	Ala	Arg	Lys	Lys	Asp	Trp	Asp	Pro	Lys	Lys	Tyr	Gly	Gly	Phe	Asp	Ser	Pro	Thr	Val	Ala	Tyr	Ser	Val	Leu	Val	Val	Ala	Lys	Val	Glu
1151-1200
	4oo8	Ala	Lys	Val	Glu	Lys	Gly	Lys	Ser	Lys	Lys	Leu	Lys	Ser	Val	Lys	Glu	Leu	Leu	Gly	Ile	Thr	Ile	Met	Glu	Arg	Ser	Ser	Phe	Glu	Lys	Asn	Pro	Ile	Asp	Phe	Leu	Glu	Ala	Lys	Gly	Tyr	Lys	Glu	Val	Lys	Lys	Asp	Leu	Ile	Ile
	5f9r	Lys	Gly	Lys	Ser	Lys	Lys	Leu	Lys	Ser	Val	Lys	Glu	Leu	Leu	Gly	Ile	Thr	Ile	Met	Glu	Arg	Ser	Ser	Phe	Glu	Lys	Asn	Pro	Ile	Asp	Phe	Leu	Glu	Ala	Lys	Gly	Tyr	Lys	Glu	Val	Lys	Lys	Asp	Leu	Ile	Ile	Lys	Leu	Pro	Lys
1201-1250
	4oo8	Lys	Leu	Pro	Lys	Tyr	Ser	Leu	Phe	Glu	Leu	Glu	Asn	Gly	Arg	Lys	Arg	Met	Leu	Ala	Ser	Ala	Gly	Glu	Leu	Gln	Lys	Gly	Asn	Glu	Leu	Ala	Leu	Pro	Ser	Lys	Tyr	Val	Asn	Phe	Leu	Tyr	Leu	Ala	Ser	His	Tyr	Glu	Lys	Leu	Lys
	5f9r	Tyr	Ser	Leu	Phe	Glu	Leu	Glu	Asn	Gly	Arg	Lys	Arg	Met	Leu	Ala	Ser	Ala	Gly	Glu	Leu	Gln	Lys	Gly	Asn	Glu	Leu	Ala	Leu	Pro	Ser	Lys	Tyr	Val	Asn	Phe	Leu	Tyr	Leu	Ala	Ser	His	Tyr	Glu	Lys	Leu	Lys	Gly	Ser	Pro	Glu
1251-1300
	4oo8	Gly	Ser	Pro	Glu	Asp	Asn	Glu	Gln	Lys	Gln	Leu	Phe	Val	Glu	Gln	His	Lys	His	Tyr	Leu	Asp	Glu	Ile	Ile	Glu	Gln	Ile	Ser	Glu	Phe	Ser	Lys	Arg	Val	Ile	Leu	Ala	Asp	Ala	Asn	Leu	Asp	Lys	Val	Leu	Ser	Ala	Tyr	Asn	Lys
	5f9r	Asp	Asn	Glu	Gln	Lys	Gln	Leu	Phe	Val	Glu	Gln	His	Lys	His	Tyr	Leu	Asp	Glu	Ile	Ile	Glu	Gln	Ile	Ser	Glu	Phe	Ser	Lys	Arg	Val	Ile	Leu	Ala	Asp	Ala	Asn	Leu	Asp	Lys	Val	Leu	Ser	Ala	Tyr	Asn	Lys	His	Arg	Asp	Lys
1301-1350
	4oo8	His	Arg	Asp	Lys	Pro	Ile	Arg	Glu	Gln	Ala	Glu	Asn	Ile	Ile	His	Leu	Phe	Thr	Leu	Thr	Asn	Leu	Gly	Ala	Pro	Ala	Ala	Phe	Lys	Tyr	Phe	Asp	Thr	Thr	Ile	Asp	Arg	Lys	Arg	Tyr	Thr	Ser	Thr	Lys	Glu	Val	Leu	Asp	Ala	Thr
	5f9r	Pro	Ile	Arg	Glu	Gln	Ala	Glu	Asn	Ile	Ile	His	Leu	Phe	Thr	Leu	Thr	Asn	Leu	Gly	Ala	Pro	Ala	Ala	Phe	Lys	Tyr	Phe	Asp	Thr	Thr	Ile	Asp	Arg	Lys	Arg	Tyr	Thr	Ser	Thr	Lys	Glu	Val	Leu	Asp	Ala	Thr	Leu	Ile	His	Gln
1351-
	4oo8	Leu	Ile	His	Gln	Ser	Ile	Thr	Gly	Leu	Tyr	Glu	Thr	Arg	Ile	Asp	Leu	Ser	Gln	Leu	Gly	Gly	Asp
	5f9r	Ser	Ile	Thr	Gly	Leu	Tyr	Glu	Thr	Arg	Ile	Asp	Leu	Ser	Gln	Leu	Gly	Gly	Asp

Cas9タンパク質のアミノ酸配列比較；4oo8（B鎖）と5f9r（A鎖；配列を4つ後ろに移動，2020年ノーベル化学賞受賞のJ.A.Doudnaらによる）

001-050
	4oo8	Gly	Ser	Gly	His	Met	Asp	Lys	Lys	Tyr	Ser	Ile	Gly	Leu	Ala	Ile	Gly	Thr	Asn	Ser	Val	Gly	Trp	Ala	Val	Ile	Thr	Asp	Glu	Tyr	Lys	Val	Pro	Ser	Lys	Lys	Phe	Lys	Val	Leu	Gly	Asn	Thr	Asp	Arg	His	Ser	Ile	Lys	Lys	Asn
	5f9r	→	→	→	→	Met	Asp	Lys	Lys	Tyr	Ser	Ile	Gly	Leu	Asp	Ile	Gly	Thr	Asn	Ser	Val	Gly	Trp	Ala	Val	Ile	Thr	Asp	Glu	Tyr	Lys	Val	Pro	Ser	Lys	Lys	Phe	Lys	Val	Leu	Gly	Asn	Thr	Asp	Arg	His	Ser	Ile	Lys	Lys	Asn
051-100
	4oo8	Leu	Ile	Gly	Ala	Leu	Leu	Phe	Asp	Ser	Gly	Glu	Thr	Ala	Glu	Ala	Thr	Arg	Leu	Lys	Arg	Thr	Ala	Arg	Arg	Arg	Tyr	Thr	Arg	Arg	Lys	Asn	Arg	Ile	Leu	Tyr	Leu	Gln	Glu	Ile	Phe	Ser	Asn	Glu	Met	Ala	Lys	Val	Asp	Asp	Ser
	5f9r	Leu	Ile	Gly	Ala	Leu	Leu	Phe	Asp	Ser	Gly	Glu	Thr	Ala	Glu	Ala	Thr	Arg	Leu	Lys	Arg	Thr	Ala	Arg	Arg	Arg	Tyr	Thr	Arg	Arg	Lys	Asn	Arg	Ile	Cys	Tyr	Leu	Gln	Glu	Ile	Phe	Ser	Asn	Glu	Met	Ala	Lys	Val	Asp	Asp	Ser
101-150
	4oo8	Phe	Phe	His	Arg	Leu	Glu	Glu	Ser	Phe	Leu	Val	Glu	Glu	Asp	Lys	Lys	His	Glu	Arg	His	Pro	Ile	Phe	Gly	Asn	Ile	Val	Asp	Glu	Val	Ala	Tyr	His	Glu	Lys	Tyr	Pro	Thr	Ile	Tyr	His	Leu	Arg	Lys	Lys	Leu	Val	Asp	Ser	Thr
	5f9r	Phe	Phe	His	Arg	Leu	Glu	Glu	Ser	Phe	Leu	Val	Glu	Glu	Asp	Lys	Lys	His	Glu	Arg	His	Pro	Ile	Phe	Gly	Asn	Ile	Val	Asp	Glu	Val	Ala	Tyr	His	Glu	Lys	Tyr	Pro	Thr	Ile	Tyr	His	Leu	Arg	Lys	Lys	Leu	Val	Asp	Ser	Thr
151-200
	4oo8	Asp	Lys	Ala	Asp	Leu	Arg	Leu	Ile	Tyr	Leu	Ala	Leu	Ala	His	Met	Ile	Lys	Phe	Arg	Gly	His	Phe	Leu	Ile	Glu	Gly	Asp	Leu	Asn	Pro	Asp	Asn	Ser	Asp	Val	Asp	Lys	Leu	Phe	Ile	Gln	Leu	Val	Gln	Thr	Tyr	Asn	Gln	Leu	Phe
	5f9r	Leu	Arg	Leu	Ile	Tyr	Leu	Ala	Leu	Ala	His	Met	Ile	Lys	Phe	Arg	Gly	His	Phe	Leu	Ile	Glu	Gly	Asp	Leu	Asn	Pro	Asp	Asn	Ser	Asp	Val	Asp	Lys	Leu	Phe	Ile	Gln	Leu	Val	Gln	Thr	Tyr	Asn	Gln	Leu	Phe	Glu	Glu	Asn	Pro
201-250
	4oo8	Glu	Glu	Asn	Pro	Ile	Asn	Ala	Ser	Gly	Val	Asp	Ala	Lys	Ala	Ile	Leu	Ser	Ala	Arg	Leu	Ser	Lys	Ser	Arg	Arg	Leu	Glu	Asn	Leu	Ile	Ala	Gln	Leu	Pro	Gly	Glu	Lys	Lys	Asn	Gly	Leu	Phe	Gly	Asn	Leu	Ile	Ala	Leu	Ser	Leu
	5f9r	Asp	Lys	Ala	Asp	Ile	Asn	Ala	Ser	Gly	Val	Asp	Ala	Lys	Ala	Ile	Leu	Ser	Ala	Arg	Leu	Ser	Lys	Ser	Arg	Arg	Leu	Glu	Asn	Leu	Ile	Ala	Gln	Leu	Pro	Gly	Glu	Lys	Lys	Asn	Gly	Leu	Phe	Gly	Asn	Leu	Ile	Ala	Leu	Ser	Leu
251-300
	4oo8	Gly	Leu	Thr	Pro	Asn	Phe	Lys	Ser	Asn	Phe	Asp	Leu	Ala	Glu	Asp	Ala	Lys	Leu	Gln	Leu	Ser	Lys	Asp	Thr	Tyr	Asp	Asp	Asp	Leu	Asp	Asn	Leu	Leu	Ala	Gln	Ile	Gly	Asp	Gln	Tyr	Ala	Asp	Leu	Phe	Leu	Ala	Ala	Lys	Asn	Leu
	5f9r	Gly	Leu	Thr	Pro	Asn	Phe	Lys	Ser	Asn	Phe	Asp	Leu	Ala	Glu	Asp	Ala	Lys	Leu	Gln	Leu	Ser	Lys	Asp	Thr	Tyr	Asp	Asp	Asp	Leu	Asp	Asn	Leu	Leu	Ala	Gln	Ile	Gly	Asp	Gln	Tyr	Ala	Asp	Leu	Phe	Leu	Ala	Ala	Lys	Asn	Leu
301-350
	4oo8	Ser	Asp	Ala	Ile	Leu	Leu	Ser	Asp	Ile	Leu	Arg	Val	Asn	Thr	Glu	Ile	Thr	Lys	Ala	Pro	Leu	Ser	Ala	Ser	Met	Ile	Lys	Arg	Tyr	Asp	Glu	His	His	Gln	Asp	Leu	Thr	Leu	Leu	Lys	Ala	Leu	Val	Arg	Gln	Gln	Leu	Pro	Glu	Lys
	5f9r	Ser	Asp	Ala	Ile	Leu	Leu	Ser	Asp	Ile	Leu	Arg	Val	Asn	Thr	Glu	Ile	Thr	Lys	Ala	Pro	Leu	Ser	Ala	Ser	Met	Ile	Lys	Arg	Tyr	Asp	Glu	His	His	Gln	Asp	Leu	Thr	Leu	Leu	Lys	Ala	Leu	Val	Arg	Gln	Gln	Leu	Pro	Glu	Lys
351-400
	4oo8	Tyr	Lys	Glu	Ile	Phe	Phe	Asp	Gln	Ser	Lys	Asn	Gly	Tyr	Ala	Gly	Tyr	Ile	Asp	Gly	Gly	Ala	Ser	Gln	Glu	Glu	Phe	Tyr	Lys	Phe	Ile	Lys	Pro	Ile	Leu	Glu	Lys	Met	Asp	Gly	Thr	Glu	Glu	Leu	Leu	Val	Lys	Leu	Asn	Arg	Glu
	5f9r	Tyr	Lys	Glu	Ile	Phe	Phe	Asp	Gln	Ser	Lys	Asn	Gly	Tyr	Ala	Gly	Tyr	Ile	Asp	Gly	Gly	Ala	Ser	Gln	Glu	Glu	Phe	Tyr	Lys	Phe	Ile	Lys	Pro	Ile	Leu	Glu	Lys	Met	Asp	Gly	Thr	Glu	Glu	Leu	Leu	Val	Lys	Leu	Asn	Arg	Glu
401-450
	4oo8	Asp	Leu	Leu	Arg	Lys	Gln	Arg	Thr	Phe	Asp	Asn	Gly	Ser	Ile	Pro	His	Gln	Ile	His	Leu	Gly	Glu	Leu	His	Ala	Ile	Leu	Arg	Arg	Gln	Glu	Asp	Phe	Tyr	Pro	Phe	Leu	Lys	Asp	Asn	Arg	Glu	Lys	Ile	Glu	Lys	Ile	Leu	Thr	Phe
	5f9r	Asp	Leu	Leu	Arg	Lys	Gln	Arg	Thr	Phe	Asp	Asn	Gly	Ser	Ile	Pro	His	Gln	Ile	His	Leu	Gly	Glu	Leu	His	Ala	Ile	Leu	Arg	Arg	Gln	Glu	Asp	Phe	Tyr	Pro	Phe	Leu	Lys	Asp	Asn	Arg	Glu	Lys	Ile	Glu	Lys	Ile	Leu	Thr	Phe
451-500
	4oo8	Arg	Ile	Pro	Tyr	Tyr	Val	Gly	Pro	Leu	Ala	Arg	Gly	Asn	Ser	Arg	Phe	Ala	Trp	Met	Thr	Arg	Lys	Ser	Glu	Glu	Thr	Ile	Thr	Pro	Trp	Asn	Phe	Glu	Glu	Val	Val	Asp	Lys	Gly	Ala	Ser	Ala	Gln	Ser	Phe	Ile	Glu	Arg	Met	Thr
	5f9r	Arg	Ile	Pro	Tyr	Tyr	Val	Gly	Pro	Leu	Ala	Arg	Gly	Asn	Ser	Arg	Phe	Ala	Trp	Met	Thr	Arg	Lys	Ser	Glu	Glu	Thr	Ile	Thr	Pro	Trp	Asn	Phe	Glu	Glu	Val	Val	Asp	Lys	Gly	Ala	Ser	Ala	Gln	Ser	Phe	Ile	Glu	Arg	Met	Thr
501-550
	4oo8	Asn	Phe	Asp	Lys	Asn	Leu	Pro	Asn	Glu	Lys	Val	Leu	Pro	Lys	His	Ser	Leu	Leu	Tyr	Glu	Tyr	Phe	Thr	Val	Tyr	Asn	Glu	Leu	Thr	Lys	Val	Lys	Tyr	Val	Thr	Glu	Gly	Met	Arg	Lys	Pro	Ala	Phe	Leu	Ser	Gly	Glu	Gln	Lys	Lys
	5f9r	Asn	Phe	Asp	Lys	Asn	Leu	Pro	Asn	Glu	Lys	Val	Leu	Pro	Lys	His	Ser	Leu	Leu	Tyr	Glu	Tyr	Phe	Thr	Val	Tyr	Asn	Glu	Leu	Thr	Lys	Val	Lys	Tyr	Val	Thr	Glu	Gly	Met	Arg	Lys	Pro	Ala	Phe	Leu	Ser	Gly	Glu	Gln	Lys	Lys
551-600
	4oo8	Ala	Ile	Val	Asp	Leu	Leu	Phe	Lys	Thr	Asn	Arg	Lys	Val	Thr	Val	Lys	Gln	Leu	Lys	Glu	Asp	Tyr	Phe	Lys	Lys	Ile	Glu	Glu	Phe	Asp	Ser	Val	Glu	Ile	Ser	Gly	Val	Glu	Asp	Arg	Phe	Asn	Ala	Ser	Leu	Gly	Thr	Tyr	His	Asp
	5f9r	Ala	Ile	Val	Asp	Leu	Leu	Phe	Lys	Thr	Asn	Arg	Lys	Val	Thr	Val	Lys	Gln	Leu	Lys	Glu	Asp	Tyr	Phe	Lys	Lys	Ile	Glu	Cys	Phe	Asp	Ser	Val	Glu	Ile	Ser	Gly	Val	Glu	Asp	Arg	Phe	Asn	Ala	Ser	Leu	Gly	Thr	Tyr	His	Asp
601-650
	4oo8	Leu	Leu	Lys	Ile	Ile	Lys	Asp	Lys	Asp	Phe	Leu	Asp	Asn	Glu	Glu	Asn	Glu	Asp	Ile	Leu	Glu	Asp	Ile	Val	Leu	Thr	Leu	Thr	Leu	Phe	Glu	Asp	Arg	Glu	Met	Ile	Glu	Glu	Arg	Leu	Lys	Thr	Tyr	Ala	His	Leu	Phe	Asp	Asp	Lys
	5f9r	Leu	Leu	Lys	Ile	Ile	Lys	Asp	Lys	Asp	Phe	Leu	Asp	Asn	Glu	Glu	Asn	Glu	Asp	Ile	Leu	Glu	Asp	Ile	Val	Leu	Thr	Leu	Thr	Leu	Phe	Glu	Asp	Arg	Glu	Met	Ile	Glu	Glu	Arg	Leu	Lys	Thr	Tyr	Ala	His	Leu	Phe	Asp	Asp	Lys
651-700
	4oo8	Val	Met	Lys	Gln	Leu	Lys	Arg	Arg	Arg	Tyr	Thr	Gly	Trp	Gly	Arg	Leu	Ser	Arg	Lys	Leu	Ile	Asn	Gly	Ile	Arg	Asp	Lys	Gln	Ser	Gly	Lys	Thr	Ile	Leu	Asp	Phe	Leu	Lys	Ser	Asp	Gly	Phe	Ala	Asn	Arg	Asn	Phe	Met	Gln	Leu
	5f9r	Val	Met	Lys	Gln	Leu	Lys	Arg	Arg	Arg	Tyr	Thr	Gly	Trp	Gly	Arg	Leu	Ser	Arg	Lys	Leu	Ile	Asn	Gly	Ile	Arg	Asp	Lys	Gln	Ser	Gly	Lys	Thr	Ile	Leu	Asp	Phe	Leu	Lys	Ser	Asp	Gly	Phe	Ala	Asn	Arg	Asn	Phe	Met	Gln	Leu
701-750
	4oo8	Ile	His	Asp	Asp	Ser	Leu	Thr	Phe	Lys	Glu	Asp	Ile	Gln	Lys	Ala	Gln	Val	Ser	Gly	Gln	Gly	Asp	Ser	Leu	His	Glu	His	Ile	Ala	Asn	Leu	Ala	Gly	Ser	Pro	Ala	Ile	Lys	Lys	Gly	Ile	Leu	Gln	Thr	Val	Lys	Val	Val	Asp	Glu
	5f9r	Ile	His	Asp	Asp	Ser	Leu	Thr	Phe	Lys	Glu	Asp	Ile	Gln	Lys	Ala	Gln	Val	Ser	Gly	Gln	Gly	Asp	Ser	Leu	His	Glu	His	Ile	Ala	Asn	Leu	Ala	Gly	Ser	Pro	Ala	Ile	Lys	Lys	Gly	Ile	Leu	Gln	Thr	Val	Lys	Val	Val	Asp	Glu
751-800
	4oo8	Leu	Val	Lys	Val	Met	Gly	Arg	His	Lys	Pro	Glu	Asn	Ile	Val	Ile	Glu	Met	Ala	Arg	Glu	Asn	Gln	Thr	Thr	Gln	Lys	Gly	Gln	Lys	Asn	Ser	Arg	Glu	Arg	Met	Lys	Arg	Ile	Glu	Glu	Gly	Ile	Lys	Glu	Leu	Gly	Ser	Gln	Ile	Leu
	5f9r	Leu	Val	Lys	Val	Met	Gly	Arg	His	Lys	Pro	Glu	Asn	Ile	Val	Ile	Glu	Met	Ala	Arg	Glu	Asn	Gln	Thr	Thr	Gln	Lys	Gly	Gln	Lys	Asn	Ser	Arg	Glu	Arg	Met	Lys	Arg	Ile	Glu	Glu	Gly	Ile	Lys	Glu	Leu	Gly	Ser	Gln	Ile	Leu
801-850
	4oo8	Lys	Glu	His	Pro	Val	Glu	Asn	Thr	Gln	Leu	Gln	Asn	Glu	Lys	Leu	Tyr	Leu	Tyr	Tyr	Leu	Gln	Asn	Gly	Arg	Asp	Met	Tyr	Val	Asp	Gln	Glu	Leu	Asp	Ile	Asn	Arg	Leu	Ser	Asp	Tyr	Asp	Val	Asp	Ala	Ile	Val	Pro	Gln	Ser	Phe
	5f9r	Lys	Glu	His	Pro	Val	Glu	Asn	Thr	Gln	Leu	Gln	Asn	Glu	Lys	Leu	Tyr	Leu	Tyr	Tyr	Leu	Gln	Asn	Gly	Arg	Asp	Met	Tyr	Val	Asp	Gln	Glu	Leu	Asp	Ile	Asn	Arg	Leu	Ser	Asp	Tyr	Asp	Val	Asp	His	Ile	Val	Pro	Gln	Ser	Phe
851-900
	4oo8	Leu	Lys	Asp	Asp	Ser	Ile	Asp	Asn	Lys	Val	Leu	Thr	Arg	Ser	Asp	Lys	Asn	Arg	Gly	Lys	Ser	Asp	Asn	Val	Pro	Ser	Glu	Glu	Val	Val	Lys	Lys	Met	Lys	Asn	Tyr	Trp	Arg	Gln	Leu	Leu	Asn	Ala	Lys	Leu	Ile	Thr	Gln	Arg	Lys
	5f9r	Leu	Lys	Asp	Asp	Ser	Ile	Asp	Asn	Lys	Val	Leu	Thr	Arg	Ser	Asp	Lys	Asn	Arg	Gly	Lys	Ser	Asp	Asn	Val	Pro	Ser	Glu	Glu	Val	Val	Lys	Lys	Met	Lys	Asn	Tyr	Trp	Arg	Gln	Leu	Leu	Asn	Ala	Lys	Leu	Ile	Thr	Gln	Arg	Lys
901-950
	4oo8	Phe	Asp	Asn	Leu	Thr	Lys	Ala	Glu	Arg	Gly	Gly	Leu	Ser	Glu	Leu	Asp	Lys	Ala	Gly	Phe	Ile	Lys	Arg	Gln	Leu	Val	Glu	Thr	Arg	Gln	Ile	Thr	Lys	His	Val	Ala	Gln	Ile	Leu	Asp	Ser	Arg	Met	Asn	Thr	Lys	Tyr	Asp	Glu	Asn
	5f9r	Phe	Asp	Asn	Leu	Thr	Lys	Ala	Glu	Arg	Gly	Gly	Leu	Ser	Glu	Leu	Asp	Lys	Ala	Gly	Phe	Ile	Lys	Arg	Gln	Leu	Val	Glu	Thr	Arg	Gln	Ile	Thr	Lys	His	Val	Ala	Gln	Ile	Leu	Asp	Ser	Arg	Met	Asn	Thr	Lys	Tyr	Asp	Glu	Asn
951-1000
	4oo8	Asp	Lys	Leu	Ile	Arg	Glu	Val	Lys	Val	Ile	Thr	Leu	Lys	Ser	Lys	Leu	Val	Ser	Asp	Phe	Arg	Lys	Asp	Phe	Gln	Phe	Tyr	Lys	Val	Arg	Glu	Ile	Asn	Asn	Tyr	His	His	Ala	His	Asp	Ala	Tyr	Leu	Asn	Ala	Val	Val	Gly	Thr	Ala
	5f9r	Asp	Lys	Leu	Ile	Arg	Glu	Val	Lys	Val	Ile	Thr	Leu	Lys	Ser	Lys	Leu	Val	Ser	Asp	Phe	Arg	Lys	Asp	Phe	Gln	Phe	Tyr	Lys	Val	Arg	Glu	Ile	Asn	Asn	Tyr	His	His	Ala	His	Asp	Ala	Tyr	Leu	Asn	Ala	Val	Val	Gly	Thr	Ala
1001-1050
	4oo8	Leu	Ile	Lys	Lys	Tyr	Pro	Lys	Leu	Glu	Ser	Glu	Phe	Val	Tyr	Gly	Asp	Tyr	Lys	Val	Tyr	Asp	Val	Arg	Lys	Met	Ile	Ala	Lys	Ser	Glu	Gln	Glu	Ile	Gly	Lys	Ala	Thr	Ala	Lys	Tyr	Phe	Phe	Tyr	Ser	Asn	Ile	Met	Asn	Phe	Phe
	5f9r	Leu	Ile	Lys	Lys	Tyr	Pro	Lys	Leu	Glu	Ser	Glu	Phe	Val	Tyr	Gly	Asp	Tyr	Lys	Val	Tyr	Asp	Val	Arg	Lys	Met	Ile	Ala	Lys	Ser	Glu	Gln	Glu	Ile	Gly	Lys	Ala	Thr	Ala	Lys	Tyr	Phe	Phe	Tyr	Ser	Asn	Ile	Met	Asn	Phe	Phe
1051-1100
	4oo8	Lys	Thr	Glu	Ile	Thr	Leu	Ala	Asn	Gly	Glu	Ile	Arg	Lys	Arg	Pro	Leu	Ile	Glu	Thr	Asn	Gly	Glu	Thr	Gly	Glu	Ile	Val	Trp	Asp	Lys	Gly	Arg	Asp	Phe	Ala	Thr	Val	Arg	Lys	Val	Leu	Ser	Met	Pro	Gln	Val	Asn	Ile	Val	Lys
	5f9r	Lys	Thr	Glu	Ile	Thr	Leu	Ala	Asn	Gly	Glu	Ile	Arg	Lys	Arg	Pro	Leu	Ile	Glu	Thr	Asn	Gly	Glu	Thr	Gly	Glu	Ile	Val	Trp	Asp	Lys	Gly	Arg	Asp	Phe	Ala	Thr	Val	Arg	Lys	Val	Leu	Ser	Met	Pro	Gln	Val	Asn	Ile	Val	Lys
1101-1150
	4oo8	Lys	Thr	Glu	Val	Gln	Thr	Gly	Gly	Phe	Ser	Lys	Glu	Ser	Ile	Leu	Pro	Lys	Arg	Asn	Ser	Asp	Lys	Leu	Ile	Ala	Arg	Lys	Lys	Asp	Trp	Asp	Pro	Lys	Lys	Tyr	Gly	Gly	Phe	Asp	Ser	Pro	Thr	Val	Ala	Tyr	Ser	Val	Leu	Val	Val
	5f9r	Lys	Thr	Glu	Val	Gln	Thr	Gly	Gly	Phe	Ser	Lys	Glu	Ser	Ile	Leu	Pro	Lys	Arg	Asn	Ser	Asp	Lys	Leu	Ile	Ala	Arg	Lys	Lys	Asp	Trp	Asp	Pro	Lys	Lys	Tyr	Gly	Gly	Phe	Asp	Ser	Pro	Thr	Val	Ala	Tyr	Ser	Val	Leu	Val	Val
1151-1200
	4oo8	Ala	Lys	Val	Glu	Lys	Gly	Lys	Ser	Lys	Lys	Leu	Lys	Ser	Val	Lys	Glu	Leu	Leu	Gly	Ile	Thr	Ile	Met	Glu	Arg	Ser	Ser	Phe	Glu	Lys	Asn	Pro	Ile	Asp	Phe	Leu	Glu	Ala	Lys	Gly	Tyr	Lys	Glu	Val	Lys	Lys	Asp	Leu	Ile	Ile
	5f9r	Ala	Lys	Val	Glu	Lys	Gly	Lys	Ser	Lys	Lys	Leu	Lys	Ser	Val	Lys	Glu	Leu	Leu	Gly	Ile	Thr	Ile	Met	Glu	Arg	Ser	Ser	Phe	Glu	Lys	Asn	Pro	Ile	Asp	Phe	Leu	Glu	Ala	Lys	Gly	Tyr	Lys	Glu	Val	Lys	Lys	Asp	Leu	Ile	Ile
1201-1250
	4oo8	Lys	Leu	Pro	Lys	Tyr	Ser	Leu	Phe	Glu	Leu	Glu	Asn	Gly	Arg	Lys	Arg	Met	Leu	Ala	Ser	Ala	Gly	Glu	Leu	Gln	Lys	Gly	Asn	Glu	Leu	Ala	Leu	Pro	Ser	Lys	Tyr	Val	Asn	Phe	Leu	Tyr	Leu	Ala	Ser	His	Tyr	Glu	Lys	Leu	Lys
	5f9r	Lys	Leu	Pro	Lys	Tyr	Ser	Leu	Phe	Glu	Leu	Glu	Asn	Gly	Arg	Lys	Arg	Met	Leu	Ala	Ser	Ala	Gly	Glu	Leu	Gln	Lys	Gly	Asn	Glu	Leu	Ala	Leu	Pro	Ser	Lys	Tyr	Val	Asn	Phe	Leu	Tyr	Leu	Ala	Ser	His	Tyr	Glu	Lys	Leu	Lys
1251-1300
	4oo8	Gly	Ser	Pro	Glu	Asp	Asn	Glu	Gln	Lys	Gln	Leu	Phe	Val	Glu	Gln	His	Lys	His	Tyr	Leu	Asp	Glu	Ile	Ile	Glu	Gln	Ile	Ser	Glu	Phe	Ser	Lys	Arg	Val	Ile	Leu	Ala	Asp	Ala	Asn	Leu	Asp	Lys	Val	Leu	Ser	Ala	Tyr	Asn	Lys
	5f9r	Gly	Ser	Pro	Glu	Asp	Asn	Glu	Gln	Lys	Gln	Leu	Phe	Val	Glu	Gln	His	Lys	His	Tyr	Leu	Asp	Glu	Ile	Ile	Glu	Gln	Ile	Ser	Glu	Phe	Ser	Lys	Arg	Val	Ile	Leu	Ala	Asp	Ala	Asn	Leu	Asp	Lys	Val	Leu	Ser	Ala	Tyr	Asn	Lys
1301-1350
	4oo8	His	Arg	Asp	Lys	Pro	Ile	Arg	Glu	Gln	Ala	Glu	Asn	Ile	Ile	His	Leu	Phe	Thr	Leu	Thr	Asn	Leu	Gly	Ala	Pro	Ala	Ala	Phe	Lys	Tyr	Phe	Asp	Thr	Thr	Ile	Asp	Arg	Lys	Arg	Tyr	Thr	Ser	Thr	Lys	Glu	Val	Leu	Asp	Ala	Thr
	5f9r	His	Arg	Asp	Lys	Pro	Ile	Arg	Glu	Gln	Ala	Glu	Asn	Ile	Ile	His	Leu	Phe	Thr	Leu	Thr	Asn	Leu	Gly	Ala	Pro	Ala	Ala	Phe	Lys	Tyr	Phe	Asp	Thr	Thr	Ile	Asp	Arg	Lys	Arg	Tyr	Thr	Ser	Thr	Lys	Glu	Val	Leu	Asp	Ala	Thr
1351-
	4oo8	Leu	Ile	His	Gln	Ser	Ile	Thr	Gly	Leu	Tyr	Glu	Thr	Arg	Ile	Asp	Leu	Ser	Gln	Leu	Gly	Gly	Asp
	5f9r	Leu	Ile	His	Gln	Ser	Ile	Thr	Gly	Leu	Tyr	Glu	Thr	Arg	Ile	Asp	Leu	Ser	Gln	Leu	Gly	Gly	Asp

画像クリックで拡大
Cas9タンパク質4oo8（B鎖）と5f9r（A鎖）のアミノ酸配列の異同（FASTA形式データから作成；PDB形式データとの違いに注）

上表の四角枠部分を中心にした拡大図

【参考】Cas9タンパク質のアミノ酸配列比較（FASTA形式データから作成）；

5b2o

5b2p

5b2q

1301-1350
	5b2o	Lys	Pro	Ile	Ser	Ile	Asp	Ile	Gln	Ile	Ser	Thr	Leu	Glu	Glu	Leu	Arg	Asn	Ile	Leu	Thr	Thr	Asn	Asn	Ile	Ala	Ala	Thr	Ala	Glu	Tyr	Tyr	Tyr	Ile	Asn	Leu	Lys	Thr	Gln	Lys	Leu	His	Glu	Tyr	Tyr	Ile	Glu	Asn	Tyr	Asn	Thr
	5b2p	Lys	Pro	Ile	Ser	Ile	Asp	Ile	Gln	Ile	Ser	Thr	Leu	Glu	Glu	Leu	Arg	Asn	Ile	Leu	Thr	Thr	Asn	Asn	Ile	Ala	Ala	Thr	Ala	Glu	Tyr	Tyr	Tyr	Ile	Asn	Leu	Lys	Thr	Gln	Lys	Leu	His	Glu	Tyr	Tyr	Ile	Glu	Asn	Tyr	Asn	Thr
	5b2q	Lys	Pro	Ile	Ser	Ile	Asp	Ile	Gln	Ile	Ser	Thr	Leu	Glu	Glu	Leu	Arg	Asn	Ile	Leu	Thr	Thr	Asn	Asn	Ile	Ala	Ala	Thr	Ala	Glu	Tyr	Tyr	Tyr	Ile	Asn	Leu	Lys	Thr	Gln	Lys	Leu	His	Glu	Tyr	Tyr	Ile	Glu	Asn	Tyr	Asn	Thr
1351-1400
	5b2o	Ala	Leu	Gly	Tyr	Lys	Lys	Tyr	Ser	Lys	Glu	Met	Glu	Phe	Leu	Arg	Ser	Leu	Ala	Tyr	Arg	Ser	Glu	Arg	Val	Lys	Ile	Lys	Ser	Ile	Asp	Asp	Val	Lys	Gln	Val	Leu	Asp	Lys	Asp	Ser	Asn	Phe	Ile	Ile	Gly	Lys	Ile	Thr	Leu	Pro
	5b2p	Ala	Leu	Gly	Tyr	Lys	Lys	Tyr	Ser	Lys	Glu	Met	Glu	Phe	Leu	Arg	Ser	Leu	Ala	Tyr	Arg	Ser	Glu	Arg	Val	Lys	Ile	Lys	Ser	Ile	Asp	Asp	Val	Lys	Gln	Val	Leu	Asp	Lys	Asp	Ser	Asn	Phe	Ile	Ile	Gly	Lys	Ile	Thr	Leu	Pro
	5b2q	Ala	Leu	Gly	Tyr	Lys	Lys	Tyr	Ser	Lys	Glu	Met	Glu	Phe	Leu	Arg	Ser	Leu	Ala	Tyr	Arg	Ser	Arg	Arg	Val	Lys	Ile	Lys	Ser	Ile	Asp	Asp	Val	Lys	Gln	Val	Leu	Asp	Lys	Asp	Ser	Asn	Phe	Ile	Ile	Gly	Lys	Ile	Thr	Leu	Pro
1401-1450
	5b2o	Phe	Lys	Lys	Glu	Trp	Gln	Arg	Leu	Tyr	Arg	Glu	Trp	Gln	Asn	Thr	Thr	Ile	Lys	Asp	Asp	Tyr	Glu	Phe	Leu	Lys	Ser	Phe	Phe	Asn	Val	Lys	Ser	Ile	Thr	Lys	Leu	His	Lys	Lys	Val	Arg	Lys	Asp	Phe	Ser	Leu	Pro	Ile	Ser	Thr
	5b2p	Phe	Lys	Lys	Glu	Trp	Gln	Arg	Leu	Tyr	Arg	Glu	Trp	Gln	Asn	Thr	Thr	Ile	Lys	Asp	Asp	Tyr	Glu	Phe	Leu	Lys	Ser	Phe	Phe	Asn	Val	Lys	Ser	Ile	Thr	Lys	Leu	His	Lys	Lys	Val	Arg	Lys	Asp	Phe	Ser	Leu	Pro	Ile	Ser	Thr
	5b2q	Phe	Lys	Lys	Glu	Trp	Gln	Arg	Leu	Tyr	Arg	Glu	Trp	Gln	Asn	Thr	Thr	Ile	Lys	Asp	Asp	Tyr	Glu	Phe	Leu	Lys	Ser	Phe	Phe	Asn	Val	Lys	Ser	Ile	Thr	Lys	Leu	His	Lys	Lys	Val	Arg	Lys	Asp	Phe	Ser	Leu	Pro	Ile	Ser	Thr
1451-1500
	5b2o	Asn	Glu	Gly	Lys	Phe	Leu	Val	Lys	Arg	Lys	Thr	Trp	Asp	Asn	Asn	Phe	Ile	Tyr	Gln	Ile	Leu	Asn	Asp	Ser	Asp	Ser	Arg	Ala	Asp	Gly	Thr	Lys	Pro	Phe	Ile	Pro	Ala	Phe	Asp	Ile	Ser	Lys	Asn	Glu	Ile	Val	Glu	Ala	Ile	Ile
	5b2p	Asn	Glu	Gly	Lys	Phe	Leu	Val	Lys	Arg	Lys	Thr	Trp	Asp	Asn	Asn	Phe	Ile	Tyr	Gln	Ile	Leu	Asn	Asp	Ser	Asp	Ser	Arg	Ala	Asp	Gly	Thr	Lys	Pro	Phe	Ile	Pro	Ala	Phe	Asp	Ile	Ser	Lys	Asn	Glu	Ile	Val	Glu	Ala	Ile	Ile
	5b2q	Asn	His	Gly	Lys	Phe	Leu	Val	Lys	Arg	Lys	Thr	Trp	Asp	Asn	Asn	Phe	Ile	Tyr	Gln	Ile	Leu	Asn	Asp	Ser	Asp	Ser	Arg	Ala	Asp	Gly	Thr	Lys	Pro	Phe	Ile	Pro	Ala	Phe	Asp	Ile	Ser	Lys	Asn	Glu	Ile	Val	Glu	Ala	Ile	Ile
1501-1550
	5b2o	Asp	Ser	Phe	Thr	Ser	Lys	Asn	Ile	Phe	Trp	Leu	Pro	Lys	Asn	Ile	Glu	Leu	Gln	Lys	Val	Asp	Asn	Lys	Asn	Ile	Phe	Ala	Ile	Asp	Thr	Ser	Lys	Trp	Phe	Glu	Val	Glu	Thr	Pro	Ser	Asp	Leu	Arg	Asp	Ile	Gly	Ile	Ala	Thr	Ile
	5b2p	Asp	Ser	Phe	Thr	Ser	Lys	Asn	Ile	Phe	Trp	Leu	Pro	Lys	Asn	Ile	Glu	Leu	Gln	Lys	Val	Asp	Asn	Lys	Asn	Ile	Phe	Ala	Ile	Asp	Thr	Ser	Lys	Trp	Phe	Glu	Val	Glu	Thr	Pro	Ser	Asp	Leu	Arg	Asp	Ile	Gly	Ile	Ala	Thr	Ile
	5b2q	Asp	Ser	Phe	Thr	Ser	Lys	Asn	Ile	Phe	Trp	Leu	Pro	Lys	Asn	Ile	Glu	Leu	Gln	Lys	Val	Asp	Asn	Lys	Asn	Ile	Phe	Ala	Ile	Asp	Thr	Ser	Lys	Trp	Phe	Glu	Val	Glu	Thr	Pro	Ser	Asp	Leu	Arg	Asp	Ile	Gly	Ile	Ala	Thr	Ile
1551-1600
	5b2o	Gln	Tyr	Lys	Ile	Asp	Asn	Asn	Ser	Arg	Pro	Lys	Val	Arg	Val	Lys	Leu	Asp	Tyr	Val	Ile	Asp	Asp	Asp	Ser	Lys	Ile	Asn	Tyr	Phe	Met	Asn	His	Ser	Leu	Leu	Lys	Ser	Arg	Tyr	Pro	Asp	Lys	Val	Leu	Glu	Ile	Leu	Lys	Gln	Ser
	5b2p	Gln	Tyr	Lys	Ile	Asp	Asn	Asn	Ser	Arg	Pro	Lys	Val	Arg	Val	Lys	Leu	Asp	Tyr	Val	Ile	Asp	Asp	Asp	Ser	Lys	Ile	Asn	Tyr	Phe	Met	Asn	His	Ser	Leu	Leu	Lys	Ser	Arg	Tyr	Pro	Asp	Lys	Val	Leu	Glu	Ile	Leu	Lys	Gln	Ser
	5b2q	Gln	Tyr	Lys	Ile	Asp	Asn	Asn	Ser	Ala	Pro	Lys	Val	Arg	Val	Lys	Leu	Asp	Tyr	Val	Ile	Asp	Asp	Asp	Ser	Lys	Ile	Asn	Tyr	Phe	Met	Asn	His	Ser	Leu	Leu	Lys	Ser	Arg	Tyr	Pro	Asp	Lys	Val	Leu	Glu	Ile	Leu	Lys	Gln	Ser
1601-
	5b2o	Thr	Ile	Ile	Glu	Phe	Glu	Ser	Ser	Gly	Phe	Asn	Lys	Thr	Ile	Lys	Glu	Met	Leu	Gly	Met	Lys	Leu	Ala	Gly	Ile	Tyr	Asn	Glu	Thr	Ser	Asn	Asn
	5b2p	Thr	Ile	Ile	Glu	Phe	Glu	Ser	Ser	Gly	Phe	Asn	Lys	Thr	Ile	Lys	Glu	Met	Leu	Gly	Met	Lys	Leu	Ala	Gly	Ile	Tyr	Asn	Glu	Thr	Ser	Asn	Asn
	5b2q	Thr	Ile	Ile	Glu	Phe	Glu	Ser	Ser	Gly	Phe	Asn	Lys	Thr	Ile	Lys	Glu	Met	Leu	Gly	Met	Lys	Leu	Ala	Gly	Ile	Tyr	Asn	Glu	Thr	Ser	Asn	Asn

画像クリックで拡大
上表の縮小画像

5fw1（TGAG PAM）・5fw2（TGAG PAM）・5fw3（TGCG PAM）

Cell（2016/02/11）

日本医療研究開発機構プレスリリース

Molecular Cell（2016/03/17）

5fw1・5fw2・5fw3

Molecular Cell（2016/03/17）

5b43

CRISPR-Cpf1

JST（2016/04/22）

Chain B（5b2r・5b2s・5b2t，5fw1・5fw2・5fw3ではChain A）　…guide RNA
5B2O:  GGGAAAUUAGGUGCGCUGGGGGUUUCAGUUGCGCCGAAAGGCGCUCUGUAAUCAUUUAAAAGUAUUUUGAACGGACCUCUGUUUGACACGUCUG
5B2P:  GGGAAAUUAGGUGCGCUGGGGGUUUCAGUUGCGCCGAAAGGCGCUCUGUAAUCAUUAAAAAGUAUUUUGAACGGACCUCUGUUUGACACGUCUG
5B2Q:  GGGAAAUUAGGUGCGCUGGGGGUUUCAGUUGCGCCGAAAGGCGCUCUGUAAUCAUUUAAAAGUAUUUUGAACGGACCUCUGUUUGACACGUCUG
5B2R:  GGAAAUUAGGUGCGCUUGGCGUUUUAGAGCUAGAAAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUG
5B2S:  GGAAAUUAGGUGCGCUUGGCGUUUUAGAGCUAGAAAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUG
5B2T:  GGAAAUUAGGUGCGCUUGCGUUUUAGAGCUAGAAAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGU G
5FW1:  GGAUAACUCAAUUUGUAAAAAAGUUUUAGAGCUAGAAAUAGCAAGUUAAAAUAAGGCUAGUCCUUAUCAACUUGAAAAAGUG
5FW2:  GGAUAACUCAAUUUGUAAAAAAGUUUUAGAGCUAGAAAUAGCAAGUUAAAAUAAGGCUAGUCCUUAUCAACUUGAAAAAGUG
5FW3:  GGAUAACUCAAUUUGUAAAAAAGUUUUAGAGCUAGAAAUAGCAAGUUAAAAUAAGGCUAGUCCUUAUCAACUUGAAAAAGUG
5B43:  AAUUUCUACUCUUGUAGAUGGAAAUUAGGUGCGCUUGGCAACC    …CRISPR-Cpf1

Chain C　…target DNA
5B2O:  CCGATACCACCCCAGCGCACCTAATTTCCC
5B2P:  CCGATATCACCCCAGCGCACCTAATTTCCC
5B2Q:  CCGATACCACCCCAGCGCACCTAATTTCCC
5B2R:  CAATCTCAGCCAAGCGCACCTAATTTCC
5B2S:  CAATCTCAGCCAAGCGCACCTAATTTCC
5B2T:  CAATCGCAGCCAAGCGCACCTAATTTCC
5FW1:  CAATCTCATTTTTTACAAATTGAGTTAT
5FW2:  CAATCTCATTTTTTACAAATTGAGTTAT
5FW3:  CAACCGCATTTTTTACAAATTGAGTTAT
5B43:  GGTTGCCAAGCGCACCTAATTTCCTAAAGGACTG    …CRISPR-Cpf1

Chain D　…non-target DNA
5B2O:  TGGTATCGG
5B2P:  TGATATCGG
5B2Q:  TGGTATCGG
5B2R:  TGAGATTG
5B2S:  TGAGATTG
5B2T:  TGCGATTG
5FW1:  AAAATGAGATTG
5FW2:  AAAATGAGATTG
5FW3:  AAAATGCGATTG
5B43:  CAGTCCTTTA    …CRISPR-Cpf1